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Warning [2] Undefined array key "lockoutexpiry" - Line: 94 - File: global.php PHP 8.0.30 (Linux)
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/global.php 94 errorHandler->error
/member.php 30 require_once
Warning [2] Undefined property: MyLanguage::$today_rel - Line: 474 - File: inc/functions.php PHP 8.0.30 (Linux)
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/inc/functions.php 474 errorHandler->error
/global.php 471 my_date
/member.php 30 require_once
Warning [2] Undefined array key "lockoutexpiry" - Line: 550 - File: global.php PHP 8.0.30 (Linux)
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/global.php 550 errorHandler->error
/member.php 30 require_once
Warning [2] Undefined property: MyLanguage::$search_button - Line: 5 - File: global.php(574) : eval()'d code PHP 8.0.30 (Linux)
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/global.php(574) : eval()'d code 5 errorHandler->error
/global.php 574 eval
/member.php 30 require_once
Warning [2] Undefined array key "avatartype" - Line: 811 - File: global.php PHP 8.0.30 (Linux)
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/global.php 811 errorHandler->error
/member.php 30 require_once
Warning [2] Undefined array key "avatartype" - Line: 811 - File: global.php PHP 8.0.30 (Linux)
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/global.php 811 errorHandler->error
/member.php 30 require_once
Warning [2] Undefined property: MyLanguage::$select2_match - Line: 15 - File: global.php(869) : eval()'d code PHP 8.0.30 (Linux)
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/global.php(869) : eval()'d code 15 errorHandler->error
/global.php 869 eval
/member.php 30 require_once
Warning [2] Undefined property: MyLanguage::$select2_matches - Line: 16 - File: global.php(869) : eval()'d code PHP 8.0.30 (Linux)
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/global.php(869) : eval()'d code 16 errorHandler->error
/global.php 869 eval
/member.php 30 require_once
Warning [2] Undefined property: MyLanguage::$select2_nomatches - Line: 17 - File: global.php(869) : eval()'d code PHP 8.0.30 (Linux)
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/global.php(869) : eval()'d code 17 errorHandler->error
/global.php 869 eval
/member.php 30 require_once
Warning [2] Undefined property: MyLanguage::$select2_inputtooshort_single - Line: 18 - File: global.php(869) : eval()'d code PHP 8.0.30 (Linux)
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/global.php(869) : eval()'d code 18 errorHandler->error
/global.php 869 eval
/member.php 30 require_once
Warning [2] Undefined property: MyLanguage::$select2_inputtooshort_plural - Line: 19 - File: global.php(869) : eval()'d code PHP 8.0.30 (Linux)
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/global.php(869) : eval()'d code 19 errorHandler->error
/global.php 869 eval
/member.php 30 require_once
Warning [2] Undefined property: MyLanguage::$select2_inputtoolong_single - Line: 20 - File: global.php(869) : eval()'d code PHP 8.0.30 (Linux)
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/global.php(869) : eval()'d code 20 errorHandler->error
/global.php 869 eval
/member.php 30 require_once
Warning [2] Undefined property: MyLanguage::$select2_inputtoolong_plural - Line: 21 - File: global.php(869) : eval()'d code PHP 8.0.30 (Linux)
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/global.php(869) : eval()'d code 21 errorHandler->error
/global.php 869 eval
/member.php 30 require_once
Warning [2] Undefined property: MyLanguage::$select2_selectiontoobig_single - Line: 22 - File: global.php(869) : eval()'d code PHP 8.0.30 (Linux)
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/global.php(869) : eval()'d code 22 errorHandler->error
/global.php 869 eval
/member.php 30 require_once
Warning [2] Undefined property: MyLanguage::$select2_selectiontoobig_plural - Line: 23 - File: global.php(869) : eval()'d code PHP 8.0.30 (Linux)
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/global.php(869) : eval()'d code 23 errorHandler->error
/global.php 869 eval
/member.php 30 require_once
Warning [2] Undefined property: MyLanguage::$select2_loadmore - Line: 24 - File: global.php(869) : eval()'d code PHP 8.0.30 (Linux)
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/global.php(869) : eval()'d code 24 errorHandler->error
/global.php 869 eval
/member.php 30 require_once
Warning [2] Undefined property: MyLanguage::$select2_searching - Line: 25 - File: global.php(869) : eval()'d code PHP 8.0.30 (Linux)
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/global.php(869) : eval()'d code 25 errorHandler->error
/global.php 869 eval
/member.php 30 require_once
Warning [2] Undefined variable $awaitingusers - Line: 34 - File: global.php(872) : eval()'d code PHP 8.0.30 (Linux)
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/global.php(872) : eval()'d code 34 errorHandler->error
/global.php 872 eval
/member.php 30 require_once
Warning [2] Undefined array key "style" - Line: 937 - File: global.php PHP 8.0.30 (Linux)
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/global.php 937 errorHandler->error
/member.php 30 require_once
Warning [2] Undefined property: MyLanguage::$lang_select_default - Line: 5197 - File: inc/functions.php PHP 8.0.30 (Linux)
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/inc/functions.php 5197 errorHandler->error
/global.php 937 build_theme_select
/member.php 30 require_once
Warning [2] Undefined array key "additionalgroups" - Line: 7361 - File: inc/functions.php PHP 8.0.30 (Linux)
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/inc/functions.php 7361 errorHandler->error
/inc/functions.php 5217 is_member
/global.php 937 build_theme_select
/member.php 30 require_once
Warning [2] Undefined property: MyLanguage::$bottomlinks_markread - Line: 15 - File: global.php(966) : eval()'d code PHP 8.0.30 (Linux)
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/global.php(966) : eval()'d code 15 errorHandler->error
/global.php 966 eval
/member.php 30 require_once
Warning [2] Undefined property: MyLanguage::$lastvisit_hidden - Line: 2552 - File: member.php PHP 8.0.30 (Linux)
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/member.php 2552 errorHandler->error
Warning [2] Undefined property: MyLanguage::$timeonline_hidden - Line: 2555 - File: member.php PHP 8.0.30 (Linux)
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/member.php 2555 errorHandler->error
Warning [2] Undefined array key "additionalgroups" - Line: 7361 - File: inc/functions.php PHP 8.0.30 (Linux)
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/inc/functions.php 7361 errorHandler->error
/member.php 2629 is_member
Warning [2] Undefined array key "showimages" - Line: 2679 - File: member.php PHP 8.0.30 (Linux)
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/member.php 2679 errorHandler->error
Warning [2] Undefined array key "additionalgroups" - Line: 7361 - File: inc/functions.php PHP 8.0.30 (Linux)
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/inc/functions.php 7361 errorHandler->error
/member.php 2629 is_member
Warning [2] Undefined array key "showimages" - Line: 2679 - File: member.php PHP 8.0.30 (Linux)
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/member.php 2679 errorHandler->error
Warning [2] Undefined array key "additionalgroups" - Line: 7361 - File: inc/functions.php PHP 8.0.30 (Linux)
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/inc/functions.php 7361 errorHandler->error
/member.php 2629 is_member
Warning [2] Undefined array key "showimages" - Line: 2679 - File: member.php PHP 8.0.30 (Linux)
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/member.php 2679 errorHandler->error
Warning [2] Undefined array key "additionalgroups" - Line: 7361 - File: inc/functions.php PHP 8.0.30 (Linux)
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/inc/functions.php 7361 errorHandler->error
/member.php 2629 is_member
Warning [2] Undefined property: MyLanguage::$tpd_percent_total - Line: 2706 - File: member.php PHP 8.0.30 (Linux)
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/member.php 2706 errorHandler->error
Warning [2] Undefined array key "additionalgroups" - Line: 7361 - File: inc/functions.php PHP 8.0.30 (Linux)
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/inc/functions.php 7361 errorHandler->error
/inc/functions_user.php 813 is_member
/member.php 2775 purgespammer_show
Warning [2] Undefined property: MyLanguage::$total_threads - Line: 52 - File: member.php(2869) : eval()'d code PHP 8.0.30 (Linux)
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/member.php(2869) : eval()'d code 52 errorHandler->error
/member.php 2869 eval



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Bio: RNA pauses RNA polymerase
Microorganisms pause their RNA polymerase at unique positions all through transcription. Besides facilitating termination, pausing also helps kind RNA buildings. Researchers have described two types of pausing: backtracked and elemental. Unlike backtrack pausing, hairpin-stabilized pausing has not been explained nonetheless. In both varieties, the pause promotes termination by inhibiting NusA binding to DNA.

This kind of RNA pausing lets scientists to discover genes which have been transcriptionally engaged and actively processing RNA. The system is delicate and it has very low background, rendering it well suited for different organisms. It demands the planning of nuclei and polymerase run-on. Its sensitivity makes it perfect for analyzing gene expression degrees in several organisms. This process can be valuable in detoxification analysis.

Global nuclear operate-on assays verified the existence of paused Pol II molecules in 2008. The sarkosyl drug blocks pause-inducing things, which permits RNA synthesis to continue. Nonetheless, the pause-inducing components can't be induced in GRO-seq, and thus a peak of signal close to promoters is a transparent sign that Pol II is paused.

The impartial RNAP2 pausing information from these scientific studies have proven that the paused RNAP2 is enriched for genes involved with mobile cycle, detoxification, and DNA repair service. These knowledge suggest that RNAP2 pausing performs a key function in regulating gene expression and chromatin framework in mammals. Even more, these studies have disclosed that the paused RNAP2 is required for detoxification, though its absence inhibits mRNA synthesis. https://www.zenearth.com